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FOR LABORATORY RESEARCH USE ONLY — not for human or veterinary use, diagnosis, treatment, or cosmetic application. Buyers must be 21+ and legally permitted to purchase research chemicals in their jurisdiction.Intended-use statement →

Thymosin Beta-4 Wholesale

Full-length thymosin β4 — 43-amino acid actin-sequestering polypeptide for tissue regeneration research, manufacturer-direct

Product Identity

CAS Number77591-33-4
SequenceAc-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH
Amino Acids43
Molecular FormulaC₂₁₂H₃₅₀N₅₆O₇₈S
Molecular Weight~4,963.5 Da
AppearanceWhite to off-white lyophilized powder
Purity≥99% by HPLC
SolubilitySoluble in sterile water or PBS
Storage2–8°C, desiccated, protect from light

Available Configurations

ConfigurationContentBest For
Standard Kit5 mg × 10 vialsTissue regeneration models; evaluation
CustomCustom mg × custom vialsOEM; private-label; bulk research

Research Background

Thymosin Beta-4 is a naturally occurring 43-amino acid polypeptide found in virtually all mammalian cells. It was originally identified as the thymic hormone responsible for T-cell maturation, but subsequent research revealed its primary intracellular function: G-actin sequestration. By maintaining the cellular actin monomer pool, Thymosin Beta-4 regulates cytoskeletal dynamics critical for cell migration, wound healing, and angiogenesis. Unlike its synthetic fragment TB-500, full-length Thymosin Beta-4 retains the complete N-terminal actin-binding domain and C-terminal regulatory regions, offering the full biological activity of the native protein.

Proposed Mechanisms

G-Actin Sequestration1:1 binding to monomeric actin (Kd ~0.7 μM) → maintains actin monomer pool for rapid filament assembly
Cell MigrationActin dynamics → lamellipodia formation in keratinocytes and endothelial cells
Anti-Apoptotic SignalingAkt/Bad pathway activation → mitochondrial outer membrane stabilization
Anti-InflammatoryReduces TNF-α, IL-6; promotes M2 macrophage polarization via NF-κB modulation

Research Focus Areas

Angiogenesis

Endothelial cell migration & capillary tube formation in wound models

Dermal Wound Healing

Keratinocyte migration; accelerated re-epithelialization

Cardiac Protection

Cardiomyocyte survival post-ischemia; Akt activation

Inflammation Resolution

NF-κB suppression; M2 macrophage polarization

Hair Follicle Cycling

Dermal papilla cell activation; anagen phase promotion

Quality Specifications

ParameterMethodCriterion
PurityHPLC at 214 nm, C18 column≥99.0% peak area
Identity (MW)ESI-MS~4,963.5 ± 1.0 Da
AppearanceVisual inspectionWhite to off-white powder
Peptide ContentAmino acid analysis≥80% net peptide
Residual MoistureKarl Fischer≤3%

Tiered Wholesale Pricing

Kit sizeCodeList price
5 mg × 10 vialsTB5US$79
10 mg × 10 vialsTB10US$146

US$79–146 per kit (10 vials), from our published wholesale price list; tiered volume discounts apply (see the tier table) — source:wholesale price list (PDF, updated 2026-08-08).

VolumeDiscountFor
1 kitList priceEvaluation; competitive analysis
5+ kits10% offInitial portfolio expansion
20+ kits20% offMid-size distributors
50+ kits30% offRegional distributors

FAQ

What is the difference between TB-500 and Thymosin Beta-4?

TB-500 is the synthetic N-terminal fragment (Ac-LKKTETQ, ~889 Da) of the full 43-amino acid Thymosin Beta-4 protein. TB-500 retains the core actin-binding motif but lacks the full C-terminal regulatory sequence. Thymosin Beta-4 is the complete native protein with broader biological activity including additional binding domains and post-translational interaction sites.

What research applications is Thymosin Beta-4 used for?

Primarily wound healing, angiogenesis, cardiac protection, and tissue regeneration research. Its actin-sequestering activity makes it a key tool in cytoskeletal dynamics studies. Also investigated in dermal repair, corneal healing, and hair follicle biology.

How should Thymosin Beta-4 be stored and reconstituted?

Reconstitute with sterile water or PBS (pH 7.0–7.4). The full-length protein is slightly more complex to handle than TB-500 due to its larger size. Aliquot single-use portions and store at -20°C. Avoid freeze-thaw cycles. Lyophilized powder: 24-month stability at 2–8°C.

Is Thymosin Beta-4 the same as Thymosin α1?

No. Thymosin α1 (28 amino acids, ~3,108 Da) and Thymosin β4 (43 amino acids, ~4,964 Da) are distinct proteins with different biological roles. Thymosin α1 is primarily immunomodulatory, while Thymosin β4 is primarily involved in actin dynamics and tissue repair.

Do you offer custom Thymosin Beta-4 configurations?

Yes. Standard kit is 5mg × 10 vials. Custom mg per vial and vial count available for qualifying wholesale orders. Contact us for a custom quotation including private-label options.

All products are manufactured for laboratory research purposes only. Thymosin Beta-4 is labeled FOR LABORATORY RESEARCH USE ONLY. Not for human or veterinary use, diagnosis, treatment, prophylaxis or any clinical, cosmetic, dietary or household application. Not a dietary supplement, pharmaceutical ingredient, cosmetic ingredient or approved drug substance. Buyers are responsible for verifying the legal status of importation and possession in their destination country.

Ready to Source Thymosin Beta-4?

Bulk pricing, tiered discounts up to 30%, COA with every order.

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